![]() These new high-performance columns deliver higher resolution and peak capacities than other bioseparation media, producing sub-2 micron results on any LC instrument. The 5µm Kinetex core-shell particle gives 3µm efficiencies at 5µm HPLC pressures, the 2.6µm core-shell particle allows for the potential of sub-2µm performance on HPLC or UHPLC systems, and the 1.7 and 1.3 µm particles offer incredible efficiency and performance gains on UHPLC systems. Phenomenex introduces Aeris - core-shell HPLC/UHPLC columns specially designed for the analysis of proteins and peptides. Phenomenex has always strived to make sure that you have all the available solutions to get the most out of your current system and method. ![]() learn how the Kinetex core-shell technology can help you rock your LC laboratory. Newer fully porous sub-2µm particles offer improved performance, but at the cost of the need for high-pressure capable instrumentation. New Kinetex HPLC and UHPLC columns for Reversed Phase, Normal Phase. Agilent Poroshell - 2.7um and 5um C8, C18, AQ, Phenyl-Hexyl, 120A and 300A (wide-pore). 5µm and 3µm fully porous particles give typical HPLC backpressures however, their efficiencies and ability to hold performance drastically decreases at higher flow rates. ACE UltraCore - 2.5um and 5um C18, Phenyl-Hexyl. Traditional fully porous particles leave much to be desired when compared to current core-shell particles. Core shell particles are less porous than fully porous particles, which leads to lower levels of band broadening and increased rates of efficiency.įor more information, please read UHPCL or Core-Shell, Which is the Winner? This article discusses the relative advantages and disadvantages of core-shell technologies and UHPLC systems further.Continuous improvement in particle technology means greater performance and productivity for all your methods. These sol-gel processing techniques incorporate nano-structuring technology, resulting in the growth of a homogenous porous shell, which envelops a compact, non-porous silica core. In order to create a core-shell particle, sol-gel processing techniques are employed initially. This article, Using Core-Shell UHPLC Columns for Improved Separation and Characterisation of Immunoglobulins and Other Large Intact Proteins, discusses some of the ways in which core-shell columns can be used in more detail. In addition to this, reducing system dwell volume and increasing detector scan rates are simple tasks which require minimal extra outlay. Yet a great deal of research and investigation has demonstrated that existing HPLC systems can be improved simply and cost-effectively with the addition of core-shell columns. Unfortunately, sub-2 particle ready equipment, which provides high speed, high efficiency analysis, generally generates pressures which exceed the standard limits of HPLC equipment. Prohibitively costly UHPLC systems are created in accordance with sub-2 particle, high pressure applications. The difference between core-shell technologies and UHPLC systems In terms of size and shape, core-shell particles are remarkably constant, which also helps to enhance separation efficiency by limiting variable analyte movement between the particles. ![]() (In order to increase efficiency it’s important to minimise sources of band broadening, such as diffusion). This is because diffusion only occurs via the porous, outer layer of the particle rather than the entire particle. Core-shell particles and high separation efficiencyĬore-shell particles’ high separation efficiency is largely due to more rapid analyte mass transfer – from the mobile phase through to the stationary phase and back again. Instead of investing in a costly UHPLC system, many labs are choosing to improve the efficiency of their existing HPLC system, increasing efficiency and reducing expenses in one fell swoop. Used in conjunction with high mobile phase flow rates, core-shell particles can reduce the amount of analysis time without impacting upon rates of separation efficiency or generating extreme back-pressures. While this may sound tricky, core-shell technologies provide a low-pressure, high efficiency solution to this particular problem. Today, the vast majority of chromatographers seek to lower costs and increase productivity simultaneously.
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